Date of Award

2019

Type

Thesis

Major

Biology

Degree Type

BS

Department

Biology

First Advisor

Kathleen Hughes

Second Advisor

Lauren King

Abstract

We studied the effects of ethynodiol diacetate on the viability of hydrogen peroxide stressed glial cells. We wanted to determine the extent in which varying concentration of ethynodiol diacetate affect cell viability of cultured glial cells from hydrogen peroxide toxicity. Based on previous studies, we predicted that the viability of hydrogen peroxide-stressed cells will increase with the presence of the progesterone, ethynodiol diacetate, and that cell viability will increase with increasing concentration of ethynodiol diacetate. Glial cells of 1x10° cells/well were introduced into a 96-well plate and allowed to grow for 24 hours in an incubator at 37°C and 5% CO2. Cells were treated with 0 nM (control), 10 nM, 100 nM, 1 uM, 10 uM ethynodiol diacetate in doublets, with half of the cells being stressed with hydrogen peroxide. Data were analyzed using a 2-way ANOVA and a Tukey’s post hoc test with a p-value of less than 0.05 being significant. Viability did not differ significantly between stressed and unstressed cells (2-way ANOVA, F9,30=1.673, P=0.211) or between cells receiving varying concentrations of ethynodiol diacetate (2-way ANOVA, F9,30=0.638 P=0.641). The results illustrate that ethynodiol diacetate, when under these treatment parameters, does not have protective effects on the glial cell cultures.

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