Date of Award
Master of Science
Alanine scanning mutagenesis was employed to determine the structural epitopes of preptin; a newly discovered polypeptide found on the proinsulin-like growth factor II. The alanine scanning mutagenesis was accomplished by the use of, cloning, affinity chromatography, gel electrophoresis, gene sequencing, and mass spectra. However, due to inconclusive results, more research is required before the epitopes can be elucidated.
Love, Jahbo M., "Design, Cloning, and Expression of Rat Preptin and Alanine Analogs" (2017). Theses and Dissertations. 291.