Date of Award
2015
Type
Thesis
Major
Biology
Degree Type
Bachelor of Science in Biology
Department
Biology
First Advisor
Dr. Monica Frazier
Second Advisor
Dr. Kathleen Hughes
Third Advisor
Dr. Cindy Ticknor
Abstract
We studied the effects of curcumin on the proliferation and viability of MDA-MB-468 breast cancer cells and its effects on the expression ofBcl-2 and p53 proteins in these cells. Based on previous studies, we predicted a dose-dependent increase in p53 (tumor suppressor) expression, and a decrease in Bcl-2 (anti-apoptotic oncoprotein) expression. Briefly, MDA-MB468 cells were seeded into 12-well plates at 2.0 * 104 cells/well and allowed to grow for 72 hrs. Cells were treated with curcumin at 0, 25, 50 and 100 pM in triplicate at time zero and cultured for another 24 hours. All data were obtained using a Muse Cell Analyzer. Proliferation and viability data were determined using an Annexin V and Dead Cell Kit, p53 expression was determined using a Multi-Colored DNA Damage Kit, and Bcl-2 expression was determined using a Bcl-2 Activation Dual Detection Kit. Results show a significant dose-dependent increase in both cell death and proliferation. However, there was no significant difference in expressions of both p53 and Bcl-2 oncoproteins across the four treatments. Future studies will investigate additional mechanisms by which curcumin induces these apoptotic effects.
Recommended Citation
Shajan, Angelin S., "The Effects Of Curcumin On The Expression Of P53 And Bcl-2 Oncoproteins On The Human Breast Carcinoma Cell Line" (2015). Theses and Dissertations. 180.
https://csuepress.columbusstate.edu/theses_dissertations/180
